Lipid Nanoformulations

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Cryo-TEM illuminates lipid nanoformulation characteristics including particle heterogeneity, size, morphology, lamellarity, and cargo encapsulation which are key for successful nanoparticle development, formulation, and manufacturing.

Direct imaging paired with quantitative & qualitative analysis of critical quality attributes (CQAs) can accelerate lipid and LNP based vaccines & therapeutics. These values can be used to inform batch-to-batch, scale-up, and process development change comparisons.

3,700+ Liposomal Samples Imaged

With over 3,700 liposomal samples imaged at NIS, we are highly experienced with lipid nanoparticles (LNPs), liposomes, and micelles.

Cryo-TEM imaging can provide quantitative & qualitative information about lipid formulations including:

Uniformity
Lamellarity
Particle size distribution
Payload distribution
Shape and overall morphology
Impurities present in the sample
PEG layer thickness

Lipid Nanoformulations We Can Image With Cryo or NS TEM

Lipid Nanoparticles (LNPs), with mRNA, RNA, DNA payloads

Lipid Nanoparticles (LNPs), with mRNA, RNA, DNA payloads

Liposomes

Liposomes

Micelles

Micelles

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Learn about Cryo-TEM Studies for Characterizing your Nanoparticles.

Explore Our Services

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Cryo-Electron Tomography (Cryo-ET)

Cryo-Electron tomography services for generating 3D volumes of heterogeneous particles, like LNPs, liposomes & complexes.

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Cryo-TEM Imaging & Analysis

Analyze critical quality attributes of your nanoparticle formulations with cryo-TEM per particle imaging & automated analysis.

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Lamellarity & Blebbing Analysis

Cryo-TEM imaging can be used to visualize lamellarity, including blebbing, to provide important structural information.

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Method Validation

Method validation services to analyze critical quality attributes for regulatory submissions.

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Morphology & Particle Shape Analysis

Complete morphology assessment, including shape & circularity, of nanoparticle formulations.

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Negative Stain TEM Imaging

Visualize gross structural features of individual particles with rapid negative stain transmission electron imaging.

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Automated Particle Classification Analysis

Quantitative, automated analysis of VLP, virus, LNP, & liposome formulation characteristics like morphology, payload & lamellarity with cryo-TEM imaging.

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Particle Size Distribution Analysis

Automated particle size distribution measurements using cryo-TEM & nsTEM imaging, including distribution curves, mean AED, mean/max Feret.

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Particle Titer Determination

Determine particle titer and ratio of empty to fully packaged capsids with cryo-TEM for development and monitoring of viral vector preparations.

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Payload Distribution & Encapsulation Efficiency Analysis

Quantitative gene & drug payload distribution in formulations including VLPs, LNPs, & liposomes with cryo-TEM imaging & automated analysis.

Frequently Asked Questions

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