Direct determination of particle morphology is a critical aspect of nanoparticle characterization. Typical analyses include particle size distribution, shape, and overall appearance of particles in a sample.
Lamellarity analysis is particularly important. Lamellarity is the number of lipid bilayers in a liposome or lipid nanoparticle (LNP). Cryo-TEM can also visualize things like bleb formations in your lipid nanoparticle (LNP). Lamellarity directly affects liposome properties and behavior for example determining encapsulation efficiency and influencing drug release. Therefore, proper characterization of nanoparticle lamellarity is crucial to understand the potential impact on bioactivity.
Our experts have many years’ experience using TEM-based methods, particularly cryo-TEM, for analyzing particle lamellarity.
Our automated methods provide reliable high-throughput image acquisition and help answer questions on size, shape, lamellarity, cargo load, and surface morphology. In addition, cryo-TEM can help evaluate the influence of storage conditions (pH, temperature, and time) on liposome or lipid nanoparticle (LNP) morphology.
See an in depth study of lamellarity & blebbing analysis on LNPs in our white paper here.
Frequently Asked Questions
Can I see an example report showing your morphology & lamellarity analysis?
How many particles do you count, or images do you analyze per sample or grid?
