July 30, 2021

Routine collection of 10,000 direct detector movies a day using Leginon

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The growth of single particle cryo-electron microscopy (cryo-EM) over the past decade has now established it as a useful technique for structural biological research.

The hardware developments in electron microscopes and image recording devices coupled with the software developments in image processing have made determination of near-atomic resolution structures by cryo-EM almost routine for well behaved samples. Given the high instrumentational, operational and maintenance costs associated with this technology, it becomes necessary to increase the overall throughput that could further accelerate throughput of users and research projects.

Citation

Kuang H, Maruthi K, Cheng A, Eng E, Potter C, Carragher B. Routine collection of 10,000 direct detector movies a day using Leginon. Microscopy and Microanalysis. 2021;27(S1):258-260. doi:10.1017/S1431927621001501

Authors

Huihui Kuang, Kashyap Maruthi, Anchi Cheng, Edward Eng, Clint Potter and Bridget Carragher

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